BioTools

Restriction enzyme digest of DNA

Restriction enzyme digest of DNA
with commercially available restriction enzymes

  Tidy Up   Reverse   Complement

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Minimum recognition size for each restriction enzyme
Type of restriction enzyme
Use only this endonuclease Commercial source
Only restriction enzymes with known bases (no N,R,Y...)
Include Type IIb restriction enzymes (Two cleaves per recognition sequence)
Include Type IIs restriction enzymes (Non-palindromic and cleavage outside of the recognition site)
When two or more input sequences are searched:
Show only endonucleases showing different restriction patterns for searched sequences.

Paste the sequence in the textbox as plain text or fasta (for several sequences).

This service recognizes 248 different cleavage patterns (from all 609 commercially available endonucleases).
This is an open source version of the restriction digest tool originally described by Bikandi et al (2004).
Version: 1.200601031




Sequence manipulation and data Restriction digest Pcr amplification
Chaos game representation Melting temperature Protein properties
 Reduce protein alphabet Seq alignment Dna to protein Skews
Microarray analysis adaptive quantification Distance among sequences
Oligonucleotide frequency Protein to dna Random seqs
Microsatellite repeats finder Find palindromes