BioTools

In silico PCR amplification

In silico PCR amplification

Sequence

Primer 11    5'- -3'
Primer 21    5'- -3'

Allow one mismatch 2

Maximum lenght of bands
  nucleotides

1 A,T,G,C and N are allowed; A+T+G+C must be 10 or more.
2 One mismatch is allowed in any position of primers.



Source code




Sequence manipulation and data Restriction digest Pcr amplification
Chaos game representation Melting temperature Protein properties
Reduce protein alphabet Seq alignment Dna to protein Skews
Microarray analysis adaptive quantification Distance among sequences
Oligonucleotide frequency Protein to dna Random seqs
Microsatellite repeats finder Find palindromes